Concerning differences between hotspots, layers and plugins: hotspots are located inside the panorama and aligned in panoramic coordinates, whereas layers/plugins are located on the screen area and aligned to its width/height and x/y.
What about playing the sound just once, try this code:
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Source code
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1
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playsound(auto,sounds/2dante.mp3|2dante.ogg,1);
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value "1" in the end means that the sound must be played just once. If you need it to be played in loops, place "0".
See here for more details:
http://www.krpano.com/plugins/soundinterface/#actions